Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pUASTLOTattB_vhhGFP4::Vkg::mCherry
(Plasmid #163929)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 163929 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUASTLOTattB
  • Backbone manufacturer
    Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014)
  • Backbone size w/o insert (bp) 8900
  • Total vector size (bp) 15500
  • Vector type
    Insect Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
  • Alt name
    GrabFP-ECM
  • Species
    D. melanogaster (fly)
  • Mutation
    G1767D
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS result had G1767D mutation towards the end of the Vkg, depositor confirms that this mutation does not affect the plasmid function. vhhGFP4 and mCherry coding sequences, separated by a short linker region, were inserted between the first and second exon in the Vkg full-length plasmid (obtained from Wang et al., 2008). This insertion site was chosen, since a viable Vkg GFP-trap line exists which carries an exogenous GFP exon at this position (Morin et al., 2001). Finally the vhhGFP4::Vkg::mCherry construct was inserted into the MCS of the pUASTLOTattB vector (Kanca et al., 2014).

pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).

vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.

Vkg plasmid:
Type IV collagens regulate BMP signalling in Drosophila. Wang X, Harris RE, Bayston LJ, Ashe HL Nature. 2008 Sep 4; 455(7209):72-7.

mCherry: Clonetech

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTLOTattB_vhhGFP4::Vkg::mCherry was a gift from Markus Affolter (Addgene plasmid # 163929 ; http://n2t.net/addgene:163929 ; RRID:Addgene_163929)
  • For your References section:

    A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila. Harmansa S, Alborelli I, Bieli D, Caussinus E, Affolter M. Elife. 2017 Apr 11;6. doi: 10.7554/eLife.22549. 10.7554/eLife.22549 PubMed 28395731