pAdEasy 2-GFP beta-gal
(Plasmid #16407)

• Purpose: (Empty Backbone)
• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: pAdEasy-2
• Backbone size (bp): 30000
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: beta-galactosidase with stuffer
• Alt name: LacZ
• Species: E. coli
• Insert Size (bp): 10000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AdEasy Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

beta-gal with stuffer (total 10kb) was cloned into pAdTrack-CMV, then recombined into pAdEasy-2.

AdEasy is a registered trademark of the Johns Hopkins University.