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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16420 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGLI3
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Alt nameGli-3
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SpeciesH. sapiens (human)
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Insert Size (bp)5085
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Entrez GeneGLI3 (a.k.a. ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SstI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GLI3 bs-2 contains the entire coding region of Gli-3 (5085bp) cloned into the SstI/SalI site of pBluescript SK.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GLI-3 bs-2 was a gift from Bert Vogelstein (Addgene plasmid # 16420 ; http://n2t.net/addgene:16420 ; RRID:Addgene_16420) -
For your References section:
The GLI gene is a member of the Kruppel family of zinc finger proteins. Kinzler KW, Ruppert JM, Bigner SH, Vogelstein B. Nature. 1988 Mar 24. 332(6162):371-4. 10.1038/332371a0 PubMed 2832761
Map uploaded by the depositor.
