pBSK-SN3 (wt p53)
(Plasmid #16421)

• Depositing Lab: Bert Vogelstein
• Publication: Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Backbone

• Vector backbone: pBluescript SK
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 1.8kb XbaI fragment of a cDNA clone containing wt p53 was cloned into the XbaI site of pBluescript SK. T7 polymerase should be used for in vitro transcription.