pBS-mPlexinA2
(Plasmid #16423)

• Depositing Lab: Jonathan Raper
• Publication: Brown et al Development. 2001 Aug . 128(16):3071-80.

Backbone

• Vector backbone: pBluescript II KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: In Situ Template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Plexin A2
• Alt name: Plexin 2
• Species: M. musculus (mouse)
• Insert Size (bp): 2200
• Mutation: None
• GenBank ID: D86949
• Entrez Gene: Plxna2 (a.k.a. 2810428A13Rik, AA589422, AW457381, OCT, PlexA2, Plxn2, mKIAA0463)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jonathan A. Raper, University of Pennsylvania
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is an improved form of the mPlexin A2 in situ template plasmid originally described in Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. PlexinA2 and semaphorin signaling during cardiac neural crest development.
Development. 2001 128(16):3071-80. Anti-sense in situ probe is generated by linearizing with BamHI and transcribing with T3 RNA polymerase.