D3 clone (p21/WAF1 promoter)
(Plasmid #16452)

• Depositing Lab: Bert Vogelstein
• Publication: el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WAF1 promoter
• Alt name: p21
• Species: H. sapiens (human)
• Insert Size (bp): 5000
• Entrez Gene: CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a ~5kb BamHI fragment of the genomic p21-WAF1 gene cloned into the BamHI site of pBluescript SK+.