hMSH2 Clone E3 (full-length)
(Plasmid #16453)

• Depositing Lab: Bert Vogelstein
• Publication: Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

Backbone

• Vector backbone: pBluescript SK
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MSH2
• Species: H. sapiens (human)
• Insert Size (bp): 3100
• Entrez Gene: MSH2 (a.k.a. COCA1, FCC1, HNPCC, HNPCC1, LCFS2, LYNCH1, MMRCS2, MSH-2, hMSH2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A full-length clone of hMSH2 cDNA was cloned into the XbaI/XhoI site of pBluescript SK.