Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pαH-S-RRAR
(Plasmid #164569)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164569 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8370
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (S-RRAR variant)
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3624
  • GenBank ID
    MN908947
  • Tags / Fusion Proteins
    • HRV3C cleavage site (before tags) (C terminal on backbone)
    • 8X His tag (C terminal on backbone)
    • 2X Strep-Tag II (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jason McLellan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-RRAR was a gift from Priyamvada Acharya (Addgene plasmid # 164569 ; http://n2t.net/addgene:164569 ; RRID:Addgene_164569)
  • For your References section:

    D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, Stalls V, Kopp MF, Henderson R, Edwards RJ, Haynes BF, Acharya P. Cell Rep. 2021 Jan 12;34(2):108630. doi: 10.1016/j.celrep.2020.108630. Epub 2020 Dec 26. 10.1016/j.celrep.2020.108630 PubMed 33417835