Stx5S-mCherry
(Plasmid #165087)

• Purpose: Expresses Stx5S-mCherry in mammalian cells
• Depositing Lab: Geert van den Bogaart
• Publication: Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 5604
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Stx5 (Short isoform)
• Alt name: STX5
• Alt name: Syntaxin 5
• Alt name: STX5A
• Species: H. sapiens (human)
• Insert Size (bp): 903
• Mutation: Lacks amino acids 1-54, enocdes short isoform of Stx5
• GenBank ID: NM_003164.5
• Entrez Gene: STX5 (a.k.a. CDG2AA, SED5, STX5A)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pEGFP-cmv
• 3′ sequencing primer: mCherry_N1

Resource Information

• Supplemental Documents:
  • Stx5S-mCherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry