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pGEX-E6AP Hect
(Plasmid #165102)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 165102 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX4T-3
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E6AP Hect
  • Alt name
    UBE3A Hect
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    731
  • Mutation
    E6AP Hect domain Amino acids 495-738 (5'QLNPY-LICG3')
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
  • Tag / Fusion Protein
    • GST from plasmid. (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-E6AP Hect was a gift from Arthur Haas (Addgene plasmid # 165102 ; http://n2t.net/addgene:165102 ; RRID:Addgene_165102)
  • For your References section:

    Measuring rates of ubiquitin chain formation as a functional readout of ligase activity. Ronchi VP, Haas AL. Methods Mol Biol. 2012;832:197-218. doi: 10.1007/978-1-61779-474-2_14. 10.1007/978-1-61779-474-2_14 PubMed 22350887