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pCMV-Neo-Bam APC 1-2644
(Plasmid #16511)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    APC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9000
  • Mutation
    Codon 2644 truncation
  • Entrez Gene
    APC (a.k.a. BTPS2, DP2, DP2.5, DP3, GS, PPP1R46)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This truncation was created using site-directed mutagenesis.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Neo-Bam APC 1-2644 was a gift from Bert Vogelstein (Addgene plasmid # 16511)
  • For your References section:

    Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW. Science. 1997 Mar 21. 275(5307):1787-90. 10.1126/science.275.5307.1787 PubMed 9065402