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CMV-Fast-1 wild-type
(Plasmid #16521)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 16521 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
    forkhead activin signal transducer-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    FOXH1 (a.k.a. FAST-1, FAST1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-Fast-1 wild-type was a gift from Bert Vogelstein (Addgene plasmid # 16521 ; ; RRID:Addgene_16521)
  • For your References section:

    Characterization of human FAST-1, a TGF beta and activin signal transducer. Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B. Mol Cell. 1998 Jul . 2(1):121-7. 10.1016/S1097-2765(00)80120-3 PubMed 9702198