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pBJ1 neo-hu FceRI gamma
(Plasmid #16540)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16540 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBJ1 neo
  • Backbone manufacturer
    Mark M Davis
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FCER1G
  • Alt name
    human FcR gamma chain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    421
  • GenBank ID
    NM_004106
  • Entrez Gene
    FCER1G (a.k.a. FCRG)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJ1 neo-hu FceRI gamma was a gift from Jean Kinet (Addgene plasmid # 16540 ; http://n2t.net/addgene:16540 ; RRID:Addgene_16540)
  • For your References section:

    Characterization and expression of the gene for the human Fc receptor gamma subunit. Definition of a new gene family. Kuster H, Thompson H, Kinet JP. J Biol Chem 1990 Apr 15;265(11):6448-52. PubMed 2138619