pBJ1 neo-hu FceRI gamma
(Plasmid #16540)

• Depositing Lab: Jean Kinet
• Publication: Kuster et al J Biol Chem 1990 Apr 15;265(11):6448-52.

Backbone

• Vector backbone: pBJ1 neo
• Backbone manufacturer: Mark M Davis
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FCER1G
• Alt name: human FcR gamma chain
• Species: H. sapiens (human)
• Insert Size (bp): 421
• GenBank ID: NM_004106
• Entrez Gene: FCER1G (a.k.a. FCRG)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert.