pRPN265NB-enh-MNase
(Plasmid #166035)

• Purpose: Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run
• Depositing Lab: Marc Timmers
• Publication: Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49. doi: 10.1093/nar/gkab038.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: GE Healthcare Life Sciences
• Backbone size w/o insert (bp): 4300
• Total vector size (bp): 5811
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Glutathione-S-Transferase/enhancer/MNase
• Species: S. japonia/camelidae/Staphylococcus aureus
• Insert Size (bp): 1512
• GenBank ID: P00644
• Promoter: lac promoter / tac promoter
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pGEX 5`
• 3′ sequencing primer: pGEX 3`

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry