pBI-EGFP-MnSOD
(Plasmid #16612)

• Depositing Lab: Bert Vogelstein
• Publication: Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Backbone

• Vector backbone: pBI-EGFP2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MnSOD
• Alt name: mitochondrial gene superoxide dismutase
• Species: H. sapiens (human)
• Insert Size (bp): 704
• Entrez Gene: SOD2 (a.k.a. GC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains full length MnSOD gene including the mitochondrial leader sequence.