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pBI-GFP-Bub1-S492Y
(Plasmid #16625)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16625 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBI-GFP
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 5099
  • Vector type
    Mammalian Expression
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BUB1 S492Y
  • Alt name
    Bub1
  • Species
    H. sapiens (human)
  • Mutation
    S492Y
  • Entrez Gene
    BUB1 (a.k.a. BUB1A, BUB1L, hBUB1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBI-GFP-Bub1 S492Y was constructed by cloning RT-PCR products from tumour line V429 into the NotI and SalI sites of pBI-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-GFP-Bub1-S492Y was a gift from Bert Vogelstein (Addgene plasmid # 16625 ; http://n2t.net/addgene:16625 ; RRID:Addgene_16625)
  • For your References section:

    Mutations of mitotic checkpoint genes in human cancers. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B. Nature. 1998 Mar 19. 392(6673):300-3. 10.1038/32688 PubMed 9521327