pCAR-IF reporter
(Plasmid
#16628)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCEP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10400
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCAR-IF
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Alt nameCAR in-frame
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Tag
/ Fusion Protein
- beta-galactosidase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer LacZ-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The reporter construct pCAR-IF (in frame) contains a hygromycin resistance gene plus a beta-galactosidase gene containing a 54-bp in-frame poly(C-A) tract at the 5' end of its coding region.
The 54 CA bases do not disrupt the beta-galactosidase reading frame. This plasmid is used as a control.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAR-IF reporter was a gift from Bert Vogelstein (Addgene plasmid # 16628 ; http://n2t.net/addgene:16628 ; RRID:Addgene_16628) -
For your References section:
A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype. Nicolaides NC, Littman SJ, Modrich P, Kinzler KW, Vogelstein B. Mol Cell Biol. 1998 Mar . 18(3):1635-41. PubMed 9488480
Map uploaded by the depositor.
