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pCI-neo Flag HAUSP
(Plasmid #16655)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16655 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HAUSP
  • Alt name
    USP7
  • Alt name
    ubiquitin specific peptidase 7
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP7 (a.k.a. HAFOUS, HAUSP, TEF1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

A fragment of human HAUSP cDNA including the entire ORF was cloned into pCI-neo at the NheI and XbaI sites. (Please note that the XbaI site is blocked by overlapping methylation). A sequence including the Flag tag was added to one of the primers so that the encoded protein contains a Flag tag at the N-terminus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo Flag HAUSP was a gift from Bert Vogelstein (Addgene plasmid # 16655 ; http://n2t.net/addgene:16655 ; RRID:Addgene_16655)
  • For your References section:

    HAUSP is required for p53 destabilization. Cummins JM, Vogelstein B. Cell Cycle. 2004 Jun . 3(6):689-92. 10.4161/cc.3.6.924 PubMed 15118411