pCI-neo Flag HAUSP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16655||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5472
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt nameubiquitin specific peptidase 7
SpeciesH. sapiens (human)
Entrez GeneUSP7 (a.k.a. HAFOUS, HAUSP, TEF1)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
A fragment of human HAUSP cDNA including the entire ORF was cloned into pCI-neo at the NheI and XbaI sites. (Please note that the XbaI site is blocked by overlapping methylation). A sequence including the Flag tag was added to one of the primers so that the encoded protein contains a Flag tag at the N-terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI-neo Flag HAUSP was a gift from Bert Vogelstein (Addgene plasmid # 16655 ; http://n2t.net/addgene:16655 ; RRID:Addgene_16655)
For your References section:HAUSP is required for p53 destabilization. Cummins JM, Vogelstein B. Cell Cycle. 2004 Jun . 3(6):689-92. 10.4161/cc.3.6.924 PubMed 15118411