pCI-neo Flag HAUSP
(Plasmid #16655)

• Depositing Lab: Bert Vogelstein
• Publication: Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

Backbone

• Vector backbone: pCI-Neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HAUSP
• Alt name: USP7
• Alt name: ubiquitin specific peptidase 7
• Species: H. sapiens (human)
• Entrez Gene: USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A fragment of human HAUSP cDNA including the entire ORF was cloned into pCI-neo at the NheI and XbaI sites. (Please note that the XbaI site is blocked by overlapping methylation). A sequence including the Flag tag was added to one of the primers so that the encoded protein contains a Flag tag at the N-terminus.