pGRG37
(Plasmid #16667)

• Depositing Lab: Nancy Craig
• Publication: McKenzie et al BMC Microbiol. 2006 . 6():39.

Backbone

• Vector backbone: pGRG37
• Backbone size w/o insert (bp): 14263
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB survival strain (from Invitrogen), 32 degrees C or lower on LB + ampicillin
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mTn7::Gateway cassette

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 (not destroyed)
• 3′ cloning site: attR2 (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • Transgene Insertion Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Grow at 32'C. Contains Gateway cassette with ccdB gene. See author's protocol for more information.

This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.