Addgene - XE124 XDsh delta DEP-GFP-CS2+ Plasmid Data

Plasmid 16785: XE124 XDsh delta DEP-GFP-CS2+

Gene/insert name:
Xdsh-delta-DEP
Alt name:
dsh
Species:
X. laevis (frog)
Entrez Gene:
dvl2 (dishevelled, dsh, dvl, xdsh)
Fusion protein or tag:
GFP
Mutation:
contains Dsh amino acids 1-433 and 503-736 (deleting 434-502)
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
4095
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-433 and 503-736 (deleting 434-502).

Linearize with Not1 and transcribe with SP6.

Notes for Use:
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.

Please note that this gene matches NM_001090627.1, NOT BC090218.1.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16785" in your Materials and Methods section.