XE128 Rfz2 TG2myc CS2+
(Plasmid #16796)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rfz2 TG2myc
• Alt name: Fzd2
• Alt name: frizzled
• Species: R. norvegicus (rat)
• Insert Size (bp): 1912
• Entrez Gene: Fzd2 (a.k.a. Fz-10, Fzd10)
• Tag / Fusion Protein:
  • c-myc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by: Jeff Brown (tags), 11/23/98.

A single c-myc epitope has been inserted into the third extracellular loop of Rfz2 by introduction of an NheI site and the epitope. The epitope
was inserted in the region of the loop where the myc and the HA tags were inserted into the Rfz1 tagged constructs--3 aa's after the PCP sequence--which
appear to be functional.

*The ClaI and XbaI sites are methylated and cannot be cut unless the construct is grown up in dam-bugs.