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Plasmid 16814: XE42 Rat frizzled-2-CS2+
  • rat frizzled 2

  • Fzd2

  • 1912

  • R. norvegicus (rat)

  • Fzd2 (Fz-10, Fzd10)

  • pCS2+
    (Search Vector Database)

  • Xenopus Expression

  • 4095

  • EcoR1

  • Yes

  • Xba1

  • Yes

  • SP6 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Randall Moon

  • UBMTA

Comments: 

Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.

Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.

Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.

Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16814" in your Materials and Methods section.