Addgene - XE42 Rat frizzled-2-CS2+ Plasmid Data

Plasmid 16814: XE42 Rat frizzled-2-CS2+

Gene/insert name:
rat frizzled 2
Alt name:
Fzd2
Insert size:
1912
Species:
R. norvegicus (rat)
Entrez Gene:
Fzd2 (Fz-10, Fzd10)
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
4095
Cloning site 5':
EcoR1
Site destroyed during cloning:
Yes
Cloning site 3':
Xba1
Site destroyed during cloning:
Yes
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.

Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.

Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.

Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16814" in your Materials and Methods section.