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XE42 Rat frizzled-2-CS2+
(Plasmid #16814)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    rat frizzled 2
  • Alt name
    Fzd2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1912
  • Entrez Gene
    Fzd2 (a.k.a. Fz-10, Fzd10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (destroyed during cloning)
  • 3′ cloning site Xba1 (destroyed during cloning)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.

Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.

Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.

Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE42 Rat frizzled-2-CS2+ was a gift from Randall Moon (Addgene plasmid # 16814)