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Plasmid 16814: XE42 Rat frizzled-2-CS2+
  • rat frizzled 2

  • Fzd2

  • 1912

  • R. norvegicus (rat)

  • Fzd2 (Fz-10, Fzd10)

  • pCS2+
    (Search Vector Database)

  • Xenopus Expression

  • 4095

  • EcoR1

  • Yes

  • Xba1

  • Yes

  • SP6 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Randall Moon

  • UBMTA

Comments: 

Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.

Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.

Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.

Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16814" in your Materials and Methods section.