XE42 Rat frizzled-2-CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16814||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namerat frizzled 2
SpeciesR. norvegicus (rat)
Insert Size (bp)1912
Entrez GeneFzd2 (a.k.a. Fz-10, Fzd10)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (destroyed during cloning)
- 3′ cloning site Xba1 (destroyed during cloning)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.
Constructed by Julia Yang-Snyder on the date of 5/19/96.
Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE42 Rat frizzled-2-CS2+ was a gift from Randall Moon (Addgene plasmid # 16814)