p_sc-eVIP
(Plasmid #168174)

• Purpose: Expresses barcoded coding genetic variants in mammalian cells
• Depositing Labs: Jesse Boehm, James T. Neal, Aviv Regev
• Publication: Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905. doi: 10.1038/s41587-021-01160-7. Epub 2022 Jan 20.

Backbone

• Vector backbone: pMT
• Backbone size w/o insert (bp): 8456
• Total vector size (bp): 9716
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TP53
• Species: H. sapiens (human)
• Insert Size (bp): 1226
• Mutation: Wildtype
• GenBank ID: NM_000546.5
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: PmeI (destroyed during cloning)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • p_sc-eVIP.gbk
  • SnapGene file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry