pBS drosophila armadillo
(Plasmid #16829)

• Depositing Lab: Eric Wieschaus
• Publication: Peifer et al Cell. 1990 Dec 21. 63(6):1167-76.

Backbone

• Vector backbone: pBluescript KS (+)
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: armadillo
• Species: D. melanogaster (fly)
• Insert Size (bp): 2700
• Entrez Gene: arm (a.k.a. Dmel_CG11579, ARM, Arm, Armadillo, CG11579, Dm Arm, Dmel\CG11579, EG:86E4.6, beta-Cat, beta-Catenin, beta-cat, beta-cat-arm, beta-catenin, betaCatenin, betacat, bgr;-Cat, dmCTNNB1, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.

T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.