pSP64T-dnWnt-11
(Plasmid #16857)

• Depositing Lab: Jim Smith
• Publication: Tada et al Development. 2000 May . 127(10):2227-38.

Backbone

• Vector backbone: pSP64T
• Backbone manufacturer: Available at Addgene (#15030)
• Backbone size w/o insert (bp): 3300
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dnWnt-11
• Alt name: Wnt11
• Alt name: Xwnt11
• Species: X. laevis (frog)
• Insert Size (bp): 850
• Mutation: amino acids 1-282. Dominant Negative.
• Entrez Gene: wnt11b.L (a.k.a. XELAEV_18038627mg, WNT11, wnt-11, wnt11b, xwnt11)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler
et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB.

For injection, linearize with BamH1 and transcribe with SP6.