XE10 Xwnt-8 CS2+
(Plasmid
#16865)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneCS2+
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Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXwnt-8
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Alt namewnt8
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Alt namexwnt-8
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SpeciesX. laevis (frog)
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Insert Size (bp)1420
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Entrez Genewnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EcoR1 insert from pGEM Xwnt-8 subcloned into EcoR1 of CS2+;
Sense RNA cut with ASP718 and transcribe with SP6.
Constructed by Jeff Brown, unpublished.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE10 Xwnt-8 CS2+ was a gift from Randall Moon (Addgene plasmid # 16865 ; http://n2t.net/addgene:16865 ; RRID:Addgene_16865)
Map uploaded by the depositor.
