XE9 Xwnt-8 CSKA
(Plasmid
#16866)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneCSKA
- Backbone size w/o insert (bp) 4120
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXwnt-8
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Alt namewnt-8
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SpeciesX. laevis (frog)
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Insert Size (bp)1420
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Entrez Genewnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
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Tag
/ Fusion Protein
- myc?
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: EcoR1 fragment of Xwnt-8 cDNA cloned behind Xenopus Cytoskeleton Actin Promoter(1.42 Kb).
Plasmid History:
Constructed by Jan Christian.
Citation: Christian and Moon, Genes and Development 7, 13 (1993)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE9 Xwnt-8 CSKA was a gift from Randall Moon (Addgene plasmid # 16866 ; http://n2t.net/addgene:16866 ; RRID:Addgene_16866)