XE7 Xwnt-8
(Plasmid #16869)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: SP64T
• Backbone size w/o insert (bp): 3000
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xwnt-8 RNA
• Alt name: xwnt-8
• Alt name: wnt-8a
• Species: X. laevis (frog)
• Insert Size (bp): 3230
• Entrez Gene: wnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (destroyed during cloning)
• 3′ cloning site: Bgl II (destroyed during cloning)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: EcoR1 insert blunted into Bgl II blunt of SP64T.
Transcription: linearize with Bam H1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian on date of 12/90.

Citation: Christian et al., Development 111, 1045 (1991)