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XE5 Xwnt-5A Hind RNA
(Plasmid #16872)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16872 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-5A Hind RNA
  • Alt name
    wnt5a
  • Alt name
    wnt
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1526
  • Mutation
    Starts with AA 14 (see comments)
  • Entrez Gene
    wnt5a.S (a.k.a. XELAEV_18003033mg, wnt-5A, wnt5a, wnt5a-B, wnt5a-a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III RF blunted into Bgl II site (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: oocyte cDNA from Melton lambda gt10 library.
Insert size: 1526 bp.
Transcription: linearize with Xba1 and transcribe with SP6.
Sense: plasmid #3 on 5/22/90.
Antisense: plasmid #2 on 5/22/90.

Plasmid History:
Constructed by Randall Moon.
Date of Plasmid and by whom: Randall Moon, 4/9/90.
Species of Sequence Origin: Xenopus.

Notes for Use:
lambda G6 Hind III RF in pGEM4 Blue (3/19/90) isolated and blunted into SP64T.

Note: Hind III cuts 9aa 5' of a met which is used for translation, residing in putative leader sequence. This Hind III site is 13 aa 3' of actual initiation met. Removal of 5' UTR and the amino terminal 13 aa's yield a more active Xwnt, but the assumption is that using the second met, and the half leader sequence yield a wild type wnt after cleavage of the shortened leader.
Citation: Moon et al., Development 119, 97 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE5 Xwnt-5A Hind RNA was a gift from Randall Moon (Addgene plasmid # 16872 ; http://n2t.net/addgene:16872 ; RRID:Addgene_16872)