Addgene - XE4 Xwnt-5A myc Plasmid Data

Plasmid 16874: XE4 Xwnt-5A myc

Gene/insert name:
full coding of Xwnt-5A
Alt name:
wnt5
Alt name:
xwnt
Insert size:
1200
Species:
X. laevis (frog)
Entrez Gene:
wnt5a-A (Xwnt-5A)
Fusion protein or tag:
MYC
Vector backbone:
pSP64T
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
3000
Cloning site 5':
Bgl II
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

Insert: full coding of Xwnt-5A.
Insert size: 1.2 Kb.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by RTM/JR on the date of 4/93.
Notes for Use:
This construct encodes full coding sequence of Xwnt-5A, without wnt UTR's.
See Xwnt 5A full-length for Genbank data.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16874" in your Materials and Methods section.