XP41 Xnot-10
(Plasmid #16968)

• Depositing Lab: Randall Moon
• Publication: Xenopus Probe Plasmids (unpublished)

Backbone

• Vector backbone: pBluescript KS+
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xnot10
• Species: X. laevis (frog)
• Insert Size (bp): 1900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full Xnot cDNA. Derived from Xnot plasmid by removing BamHI-HindIII piece and putting it in KS. Note insert is backwards. Use EcoRV and T7 for antisense in situ probe.

Plasmid History:
From: David Kimelman.