XP31 Vox
(Plasmid #16972)

• Depositing Lab: Randall Moon
• Publication: Schmidt et al Development. 1996 Jun;122(6):1711-21. doi: 10.1242/dev.122.6.1711.

Backbone

• Vector backbone: pBluescript SK
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vox
• Alt name: Vox-15
• Alt name: Vent-2
• Species: X. laevis (frog)
• Entrez Gene: ventx2.1.L (a.k.a. XELAEV_18034617mg, Xbr-1a, Xvr-11b, ventx2.1-a, ventx2.1-b, vox, vox1, xom, xvent-2, xvent-2b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transcription: antisense probe is made by digesting with BamHI and transcribing with T7.

Plasmid History:
Constructed by Jennifer Schmidt in 1996.

Ref: Schmidt et al, 1996, Development 122:1711-1721.