XP4 Xwnt-5A
(Plasmid #16987)

• Depositing Lab: Randall Moon
• Publication: Xenopus Probe Plasmids (unpublished)

Backbone

• Vector backbone: pGem
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xwnt-5A
• Alt name: Wnt-5A
• Species: X. laevis (frog)
• Insert Size (bp): 380
• Entrez Gene: wnt5a.L (a.k.a. Xwnt-5A, wnt-5a, wnt5a-a, wnt5a-b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: PCR clone with wnt primers. Insert size: 380 bp.
Probe cut with BamH1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian and Randall Moon.

Note for Use:
Cut with BamH1, transcribe with SP6, yielding 165 nt probe, 142 nt protected fragment. Hybridize 45 degrees C with 50,000 cpm probe (higher probe yields unacceptable background). Run 8% sequencing gel 1.5 hours.