XP2 Xwnt-3A
(Plasmid
#16989)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGem2
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXwnt-3A
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Alt nameWnt-3A
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Alt namewnt3-a
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SpeciesX. laevis (frog)
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Insert Size (bp)1870
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Entrez Genewnt3 (a.k.a. Xwnt-3, Xwnt3, wnt-3, wnt3-A, wnt3l)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: cDNA. Antisense cut with Cla1 and transcribe with T7. Sense probe cut with Nco1 and transcibe with SP6.
Plasmid History:
Constructed by S. Wolda.
Notes for Use:
Another name for this clone is FC.
Citation: Wolda et al., Developmental Biology 155, (1993).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XP2 Xwnt-3A was a gift from Randall Moon (Addgene plasmid # 16989 ; http://n2t.net/addgene:16989 ; RRID:Addgene_16989)
Map uploaded by the depositor.