XP1 Xwnt-1
(Plasmid #16990)

• Depositing Lab: Randall Moon
• Publication: Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Backbone

• Vector backbone: pGem2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xwnt-1
• Alt name: Wnt-1
• Alt name: wnt1-A
• Species: X. laevis (frog)
• Insert Size (bp): 2000
• Entrez Gene: wnt1.L (a.k.a. XELAEV_18013314mg, Xint-1, Xwnt1, int-1, int1, pxwnt-1, wnt-1, wnt1, wnt1-a)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: cDNA from stage 17 lambda gt10 of Melton library.
Antisense probe: cut with Hind III and transcribe with Sp6.
Sense probe: cut with Sph1 and transcribe with T7.

Plasmid History:
Constructed by N. Edelstein and R. Moon on date of 8/92.

Citation: Wolda et al., Developmental Biology 155, (1993).