XE238 hVangl2/Strabismus pCS2P+
(Plasmid #16992)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pCS2P+
• Backbone size w/o insert (bp): 4100
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vangl2/Strabismus
• Alt name: vangl
• Species: H. sapiens (human)
• Entrez Gene: VANGL2 (a.k.a. LPP1, LTAP, STB1, STBM, STBM1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: NA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a wild type human VANGL2/Strabismuus, cloned into pCS2P+. Injected RNA in zebrafish embryos gives the expected convergent/extension phenotype (Chris Thorpe, unpub. observations).

Reference: Made by Yan Zhang by PCR for RTM. Cloned as a Cla1-Xho1 insert.