pDONR221 CARD8 ZUC
(Plasmid
#169964)
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PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Vector typeGateway cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARD8
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SpeciesH. sapiens (human)
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Entrez GeneCARD8 (a.k.a. CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 F
- 3′ sequencing primer M13 R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221 CARD8 ZUC was a gift from Daniel Bachovchin (Addgene plasmid # 169964 ; http://n2t.net/addgene:169964 ; RRID:Addgene_169964) -
For your References section:
Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H. Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 10.1016/j.immuni.2021.04.024. Epub 2021 May 20. 10.1016/j.immuni.2021.04.024 PubMed 34019797
