XE40 pSP64T-PABP (Clone 63)
(Plasmid
#17019)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 17019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepSP64T
- Backbone size w/o insert (bp) 3000
-
Vector typeXenopus Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePoly(A) binding protein
-
Alt namePABP
-
SpeciesX. laevis (frog)
-
Insert Size (bp)2200
-
Entrez Genepabpc1.S (a.k.a. XELAEV_18033915mg, pab1, pabp, pabp1, pabpc1, pabpc1-a, pabpc1-b, pabpc1.L, pabpc2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (not destroyed)
- 3′ cloning site Bgl II (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: Poly(A) binding protein clone w/ Beta-globin NC regions made from clones E&F (Zelus et al, 1989), subcloned with Bgl II linkers.
Transcription: linearize with XbaI or SmaI and transcribe with SP6.
Plasmid History:
Constructed by Bruce Zelus.
Reference Zelus et al. 1989. Molec. Cell. Biol. 9:2756-2760.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
XE40 pSP64T-PABP (Clone 63) was a gift from Randall Moon (Addgene plasmid # 17019 ; http://n2t.net/addgene:17019 ; RRID:Addgene_17019)
Map uploaded by the depositor.