Addgene - ZE26 Stbm-N-6myc-pCS2+ Plasmid Data

Plasmid 17068: ZE26 Stbm-N-6myc-pCS2+

Gene/insert name:
Stbm
Alt name:
strabismus
Species:
D. rerio (zebrafish)
Entrez Gene:
vangl2 (stbm, tri, vang)
Fusion protein or tag:
Myc6
Terminal:
N terminal on backbone
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Zebrafish Expression
Backbone size w/o insert (bp):
4100
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
XbaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert.

For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.

Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17068" in your Materials and Methods section.