ZE33 Prickle-1-GFPnopren-pCS2+
(Plasmid #17074)

• Depositing Lab: Randall Moon
• Publication: Zebrafish Expression Plasmids (unpublished)

Backbone

• Vector backbone: CS2+GFP-BB
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Prickle-1
• Alt name: pk1
• Species: D. rerio (zebrafish)
• Mutation: Pk1GFPnopren deletes the C-terminal six amino acids containing the putative prenylation site.
• Entrez Gene: prickle1a (a.k.a. fk71b09, pk1, prickle1, wu:fk71b09)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR.

Species of Sequence Origin: zebrafish.

Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.

Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).