ZE37 Nlk1(K117M)-pCS2+
(Plasmid
#17078)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNlk1
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Alt namenlk
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Alt namenemo-like kinase
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1425
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MutationK117M. Kinase dead mutant of NLK1.
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GenBank IDAY562552
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Entrez Genenlk1 (a.k.a. nlk, nlkb, zgc:111875)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an kinase dead mutant of NLK1, analogous to K155M mutant of mouse NLK.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZE37 Nlk1(K117M)-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17078 ; http://n2t.net/addgene:17078 ; RRID:Addgene_17078)