pDONRp5-p2 GFP-NShroom3-iLID
(Plasmid #170976)

• Purpose: N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.
• Depositing Lab: Miki Ebisuya
• Publication: Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi: 10.1038/s41467-022-33115-0.

Backbone

• Vector backbone: pDONR221 p5-p2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2553
• Total vector size (bp): 8070
• Vector type: Gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NShroom3
• Species: M. musculus (mouse)
• Insert Size (bp): 4161
• Mutation: From isoform 2, deleted amino acids 1389 - 1805, mutations: G1226E, E1227K, P1242L, R1276L
• GenBank ID: XP_006535031.1
• Entrez Gene: Shroom3 (a.k.a. AL022960, D5Ertd287, D5Ertd287e, Sh, Shr, Shrm, Shrm3)
• Tags / Fusion Proteins:
  • eGFP (N terminal on insert)
  • iLID (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 fwd: GTAAAACGACGGCCAGT
• 3′ sequencing primer: M13 rev: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • pDONRp5p2GFP-NShroom3-iLID.gbk
• A portion of this plasmid was derived from a plasmid made by: Original mShroom3 sequence was a gift from T. Nishimura, from M.Takeichi lab. iLID sequence was a gift from H. Janovjak lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.20.440475 for bioRxiv preprint.