pAAV GFAP jGCaMP7b
(Plasmid #171118)

• Purpose: Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter
• Depositing Lab: Thomas Oertner
• Publication: Lohr et al Front Cell Neurosci. 2021 Jun 11;15:690147. doi: 10.3389/fncel.2021.690147. eCollection 2021.

Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 6296
• Total vector size (bp): 7684
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: jGCaMP7b
• Alt name: GCaMP3-T302P R303P A317L M374Y D380Y T381R S383T R392G GCaMP3 variant 1561
• Alt name: Janelia GCaMP7b
• Species: R. norvegicus (rat), G. gallus (chicken), Synthetic; A.victoria (Jelly fish)
• Insert Size (bp): 1388
• Promoter: GFAP
• Tags / Fusion Proteins:
  • 6 His Tag (N terminal on insert)
  • T7 Tag (N terminal on insert)
  • Xpress Tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GFAP for: 5´-gccagtctagcccactccttc
• 3′ sequencing primer: WPRE rev: 5´-agctgacaggtggtggcaat

Resource Information

• Supplemental Documents:
  • pAAV GFAP jGCaMP7b.gb
• A portion of this plasmid was derived from a plasmid made by: jGCaMP7b is from Douglas Kim, GENIE Project Addgene #104489 The promoter is from Teresa Alonso
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry