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M16 pCS-twhh-bGFP
(Plasmid #17153)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17153 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    twhh promoter
  • Alt name
    tiggy-winkle hedgehog
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Entrez Gene
    shhb (a.k.a. twh, twhh)
  • Tag / Fusion Protein
    • bGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Insert: twhh promoter (5.2 Kb), bGFP (1 Kb)

Plasmid History:

The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP.

Notes for Use:
Gives notochord-specific expression of GFP.
Citation: Du et al., 1997, J. Cell Biology.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M16 pCS-twhh-bGFP was a gift from Randall Moon (Addgene plasmid # 17153 ; ; RRID:Addgene_17153)