|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17153||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nametwhh promoter
Alt nametiggy-winkle hedgehog
SpeciesD. rerio (zebrafish)
Insert Size (bp)5200
Entrez Geneshhb (a.k.a. twh, twhh)
/ Fusion Protein
- bGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
Insert: twhh promoter (5.2 Kb), bGFP (1 Kb)
The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP.
Notes for Use:
Gives notochord-specific expression of GFP.
Citation: Du et al., 1997, J. Cell Biology.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M16 pCS-twhh-bGFP was a gift from Randall Moon (Addgene plasmid # 17153 ; http://n2t.net/addgene:17153 ; RRID:Addgene_17153)
Map uploaded by the depositor.