pCK-Drosha-FLAG(delta6)
(Plasmid
#171532)
-
PurposeEncodes mutant human Drosha protein
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCK-Drosha-FLAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDROSHA
-
SpeciesH. sapiens (human)
-
Mutationsmall deletions of Drosha_1271A to 1276G_
-
Entrez GeneDROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note DROSHA contains 4 mutations-S30P, A135V, S201G and S321L. These do not affect function as they are found in the original pCK-Drosha-FLAG (Lee et al, 2003, Nature).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCK-Drosha-FLAG(delta6) was a gift from Yan Zeng (Addgene plasmid # 171532 ; http://n2t.net/addgene:171532 ; RRID:Addgene_171532) -
For your References section:
The insertion in the double-stranded RNA binding domain of human Drosha is important for its function. Zhang X, Li P, Lin J, Huang H, Yin B, Zeng Y. Biochim Biophys Acta Gene Regul Mech. 2017 Dec;1860(12):1179-1188. doi: 10.1016/j.bbagrm.2017.10.004. Epub 2017 Nov 3. 10.1016/j.bbagrm.2017.10.004 PubMed 29109067
