M18 pCS-twhh-nBeta gal Vec
(Plasmid #17155)

• Depositing Lab: Randall Moon
• Publication: Other Plasmids (unpublished)

Backbone

• Vector backbone: pCS-NBeta gal

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: twhh promoter
• Alt name: tiggy-winkle hedgehog
• Species: D. rerio (zebrafish)
• Insert Size (bp): 5200
• Entrez Gene: shhb (a.k.a. twh, twhh)
• Tag / Fusion Protein:
  • nuclear beta-gal (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Parent vector: pCS-twhh-nBeta gal.
Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb)

Plasmid History:
Source: zebrafish.

Notes for Use:

To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence.

Citation: Du et al., 1997, J. Cell Biology.