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M18 pCS-twhh-nBeta gal Vec
(Plasmid #17155)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17155 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    twhh promoter
  • Alt name
    tiggy-winkle hedgehog
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5200
  • Entrez Gene
    shhb (a.k.a. twh, twhh)
  • Tag / Fusion Protein
    • nuclear beta-gal (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Parent vector: pCS-twhh-nBeta gal.
Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb)

Plasmid History:
Source: zebrafish.

Notes for Use:

To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence.

Citation: Du et al., 1997, J. Cell Biology.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M18 pCS-twhh-nBeta gal Vec was a gift from Randall Moon (Addgene plasmid # 17155 ; http://n2t.net/addgene:17155 ; RRID:Addgene_17155)