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M64 pGEX4T-1-TEV-human beta cat domain N(1-137)
(Plasmid #17205)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    beta catenin
  • Alt name
    b-catenin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    411
  • Mutation
    amino-terminal residues 1-137
  • Entrez Gene
    CTNNB1 (a.k.a. OK/SW-cl.35, CTNNB, MRD19, armadillo)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI / XhoI (destroyed during cloning)
  • 5′ sequencing primer pGEX 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Description: This is an amino terminal human beta catenin residues 1-137 downstream of the GST fusion. Cloned as a BamHI-SalI fragment into BamHI and XhoI sites.

Reference: Made by Ken-Ichi Takemaru.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M64 pGEX4T-1-TEV-human beta cat domain N(1-137) was a gift from Randall Moon (Addgene plasmid # 17205)