Addgene - pOKD4 Plasmid Data

Plasmid 17214: pOKD4

Gene/insert name:
None
Vector backbone:
pOKD4
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size (bp):
3934
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Hao Wu
Terms and Licenses:
MTA

Comments:

Bacterial co-expression vectors to facilitate the production of substantial amounts of recombinant multiprotein complexes for biochemical, biophysical, and structural studies. pOKD4 (kanamycin-resistant) and pOKD5 (ampicillin-resistant) are derivatives of pACYC177 cloning and pET26b expression vectors. As a result, pOKD4 and pOKD5 are T7-based expression plasmids containing the p15A origin of replication. This feature permits either pOKD4 or pOKD5 to co-exist in the same bacterial cell with most Escherichia coli expression vectors including the popular pET expression vectors.

Full sequence is approximated. See article for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: General co-expression vectors for the overexpression of heterodimeric protein complexes in Escherichia coli. Dzivenu et al (Protein Expr Purif. 2004 Nov . 38(1):1-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17214" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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