Addgene - pCGLex Plasmid Data

Plasmid 17264: pCGLex

Gene/insert name:
None
Fusion protein or tag:
LexA
Terminal:
N terminal on backbone
Vector backbone:
pPC86
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size (bp):
6160
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SacII
Site destroyed during cloning:
No
5' sequencing primer:
CGTCAGCAGAGCTTCACCATTG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
TRP1
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Stuart Schreiber
Terms and Licenses:
MTA

Comments:

ref for pPC86: Chevray, P. M. & Nathans, D. (1992) Proc. Natl. Acad. Sci. USA 89, 5789-5793; ref for pEG202: Golemis, E. A., Gyuris, J. & Brent, R. (1996) in Current Protocols in Molecular Biology, eds. Ausubel, F. M., Brent, R., Kingston, R., Moore, D., Seidman, J., Smith, J. A. & Struhl, K. (Wiley, New York), p. 20.1.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. Huang et al (Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17264" in your Materials and Methods section.