pTSA-C
(Plasmid #17329)

• Depositing Lab: Takeshi Sano
• Publication: Reznik et al Bioconjug Chem. . 12(6):1000-4.

Backbone

• Vector backbone: pET-3a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: streptavidin
• Alt name: Stv-C
• Species: Streptomyces avidinii
• Insert Size (bp): 400
• Mutation: Truncated to include only amino acids 16-133 of mature streptavidin. Following Val133, a six-residue immobilization sequence (Gly-Gly-Ser-Gly-Cys-Pro) was added.
• GenBank ID: X03591

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.