|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17329||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)400
MutationTruncated to include only amino acids 16-133 of mature streptavidin. Following Val133, a six-residue immobilization sequence (Gly-Gly-Ser-Gly-Cys-Pro) was added.
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTSA-C was a gift from Takeshi Sano (Addgene plasmid # 17329)
For your References section:A streptavidin mutant useful for directed immobilization on solid surfaces. Reznik GO, Vajda S, Cantor CR, Sano T. Bioconjug Chem. . 12(6):1000-4. 10.1021/bc015507t PubMed 11716692