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pTSA-C
(Plasmid #17329)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 17329 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    streptavidin
  • Alt name
    Stv-C
  • Species
    Streptomyces avidinii
  • Insert Size (bp)
    400
  • Mutation
    Truncated to include only amino acids 16-133 of mature streptavidin. Following Val133, a six-residue immobilization sequence (Gly-Gly-Ser-Gly-Cys-Pro) was added.
  • GenBank ID
    X03591

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTSA-C was a gift from Takeshi Sano (Addgene plasmid # 17329)
  • For your References section:

    A streptavidin mutant useful for directed immobilization on solid surfaces. Reznik GO, Vajda S, Cantor CR, Sano T. Bioconjug Chem. . 12(6):1000-4. 10.1021/bc015507t PubMed 11716692